The regulatory relationship between RagA and Nprl2 in Drosophila gut development / 生物工程学报

The gastrointestinal tract is the largest digestive organ and the largest immune organ and detoxification organ, which is vital to the health of the body. Drosophila is a classic model organism, and its gut is highly similar to mammalian gut in terms of cell composition and genetic regulation, therefore can be used as a good model for studying gut development. target of rapmaycin complex 1 (TORC1) is a key factor regulating cellular metabolism. Nprl2 inhibits TORC1 activity by reducing Rag GTPase activity. Previous studies have found that nprl2 mutated Drosophila showed aging-related phenotypes such as enlarged foregastric and reduced lifespan, which were caused by over-activation of TORC1. In order to explore the role of Rag GTPase in the developmental defects of the gut of nprl2 mutated Drosophila, we used genetic hybridization combined with immunofluorescence to study the intestinal morphology and intestinal cell composition of RagA knockdown and nprl2 mutated Drosophila. The results showed that RagA knockdown alone could induce intestinal thickening and forestomach enlargement, suggesting that RagA also plays an important role in intestinal development. Knockdown of RagA rescued the phenotype of intestinal thinning and decreased secretory cells in nprl2 mutants, suggesting that Nprl2 may regulate the differentiation and morphology of intestinal cells by acting on RagA. Knockdown of RagA did not rescue the enlarged forestomach phenotype in nprl2 mutants, suggesting that Nprl2 may regulate forestomach development and intestinal digestive function through a mechanism independent of Rag GTPase.

Relationship between breakfast eating frequency and health-risk behavior of high school students in Chongqing / 中国学校卫生

Objective@#To investigate the relationship between breakfast eating frequency and health-risk behavior of high school students in Chongqing, and provide a reference for conducting students to get a good habit of eating breakfast everyday.@*Methods@#A total of 3 283 high school students in Chongqing were selected to participate in a questionnarie by using convenience sampling method. Logistic regression analysis were condueted to analyze the influences made by the unhealty eating behaviors on eating frequency of breakfast.@*Results@#There were 2 064(69.2%) students eating breakfast everyday, 1 219(37.1%) students having no breakfast or eating breakfast sometimes. There were significant differences between different gender, grade, native place, drinking energy drink, smoking, drinking alcohol, gambling, internet addiction students rate of unhealthy breakfast eating frequency(χ2=6.80, 53.97, 8.10, 23.79, 7.89, 16.31, 3.93, 17.96, P<0.05), the males (36.1%) was beyond the females(33.3%), the energy drink drinkers(41.2%) was beyond the non-drinkers(31.7%), the smokers (40.9%) was beyond the non-smokers(34.4%), the alcohol drinkers(40.3%) was beyond the non-drinkers(33.3%), the gamblers(39.7%) was beyond the non-gamblers(34.3%), those having internet addictions (40.0%) was beyond those having no addictions(33.0%). Drinking energy drink(OR=1.32, 95%CI=1.12-1.55), drinking alcohol(OR=1.24, 95%CI=1.02-1.51), internet addiction(OR=1.42, 95%CI=1.19-1.68) were positively correlated with unhealthy breakfast eating frequency of high school students(P<0.05).@*Conclusion@#Social, families and schools should strengthen the guidance of high school students to develop the habit of eating breakfast every day, and advoid the clustering of skipping breakfast and health-risk behaviors.

Evaluation of Septin9, SDC2, TFPI2 methylation and fecal occult blood in the screening of large intestine lesions / 中华检验医学杂志

Objective:To investigate the clinical application value of peripheral blood methylation Septin9 gene in peripheral blood combined with SDC2 gene methylation and TFPI2 gene methylation and fecal occult blood test in colorectal lesion screening.Methods:Through random-selection method. Seventy-five patients with colorectal cancer and 50 patients with advanced adenoma who were treated in our hospital from January 2017 to June 2019 were enrolled as the colorectal cancer group and the advanced adenocarcinoma group, respectively, and the results of the negative test were used as controls Methylation-specific PCR (MSP) was used to detect the methylation status of the Septin9, SDC2 and TFPI2 genes in all patients and the fecal occult blood test for colorectal cancer and advanced adenocarcinoma. In addition, the receiver operating characteristic curve (ROC curve) was drawn to compare the diagnostic value of combined detection and individual detection.Results:The positive rate of Septin9, SDC2, TFPI2 methylation and FOBT in colorectal cancer group was 53.04% (61/115 cases), 55.65% (64/115 cases), 59.13% (68/115 cases) and 26.09% (30/115 cases), respectively. The positive detection rates of Septin9, SDC2, TFPI2 methylation and FOBT in the advanced adenoma group were 33.33% (30/90 cases), 50% (45/90 cases), 40.00% (36/90 cases) and 17.78% (16/90 cases), respectively. The detection sensitivity (82.7%) was higher than the sensitivity of each single test and combined detection of SDC2 and TFPI2 gene methylation: Septin9 methylation (66.7%), SDC2 methylation (69.3%), TFPI2 methylation (73.3%), FOBT (26.7%), SDC2 and TFPI2 methylation combined detection (77.3%), while maintaining a high specificity (80.0%). Septin9, SDC2, TFPI2 gene methylation joint inspection screening advanced adenomas detection sensitivity (70.0%) was higher than the sensitivity of each single test and combined detection of SDC2 and TFPI2 gene methylation: (40.0%), SDC2 Septin9 gene methylation gene methylation (64.0%), TFPI2 methylation (50.0%), FOBT (18.0%), SDC2 and TFPI2 gene methylation detection (64.0%), and also maintained a high specificity (82.5%). ROC curve analysis showed that the area under the ROC curve of the three gene methylation combined screening for colorectal cancer was significantly higher than that of Septin9, SDC2, TFPI2 gene methylation, FOBT alone, and SDC2 combined with TFPI2 gene methylation detection (0.684, 0.765, 0.623, 0.796 and 0.566), and the difference was statistically significant ( P<0.05); and the area under the ROC curve of three gene methylation combined screening screening for advanced adenomas was significantly higher than the area under the ROC curve of Septin9, SDC2, TFPI2 gene methylation, FOBT alone, and SDC2 combined with TFPI2 gene methylation (0.568, 0.685, 0.535, 0.723 and 0.489), and the difference was statistically significant ( P<0.05). Conclusions:Combined detection of Septin9, SDC2 and TFPI2 gene methylation can improve the sensitivity of colorectal lesion diagnosis and maintain high specificity. It has the clinical application value for early screening and diagnosis and treatment of colorectal cancer.

The influence of negative suction during LASIK on content of retinal amino acids in rabbit / 眼科研究

Background Whether negative suction during excimer laser in situ keratomileusis(LASIK) affect the structure and function of retina or not is in controversy,but it seems that temporary hypertention induced by negative suction is a key factor of impairment of retina in LASIK.ObjectiveThis study attempts to study the influence of transient high intraocular pressure (IOP) during LASIK on the contents of retinal amino acid.MethodsThe both eyes of 45 New Zealand white rabbits were suctioned for different periods (20s,45s,3min) with negative pressure generator during the LASIK to make the instantaneous high IOP models,and LASIK without negative suction was performed in the both eyes of 15 rabbits in the control group.The changes of the contents of retinal amino acids were evaluated with High Performance Liquid Chromatography(HPLC) at 0,7,10,14 and 28 days postoperatively and compared with those of control group.ResultsThere were no statistically significant differences in the contents of retinal amino acids among different time points after operation in negative suction for 20s group and 45s group,respectively(P＞0.05).At negative suction for 3minuts,the content of glutamic acid in retina was significantly increased in comparison with control group in 7,10,14 and 28 days (P＜0.05),and no statistically significant difference was seen in the contents of glutamic acid at postoperative instant group compared with control group(P＞0.05).A statistically significant difference in the contents of glutamine,tryptophan,phenylalanine was revealed among postoperative 10 days,14 days and 28 days groups comparison with control group (P＜0.05).ConclusionThe acute IOP elevation caused by negative suction during LASIK results in the reversible increase of retinal amino acids.The duration of negative suction time influent the reconstruction of retinal structure.